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Not all reactions of a biochemical pathway are spontaneous. Biochemical energy coupling is the way forward if all relevant biochemical reactions must proceed. Energy from thermodynamically favourable processes drive enrgy requiring reactions.

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Polymerase chain reaction (PCR) is an important method in molecular biology with application in medicine and diagnostics, forensics, DNA fingerprinting etc.

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Advances in molecular biology have seen the development of various deoxyribonucleic acid (DNA) sequencing techniques such as  Next - Generation Sequencing that employs a number of different modern DNA sequencing technologies. The human genome as well as the genome of several other organisms have now been completely sequenced.

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